The Centre operates on a 24-hour basis. The team includes three lecturers in veterinary science, a veterinary military assistant and a group of veterinary students, specifically trained in veterinary toxicology. These students take part in the activities and management of the Centre and, once trained, become responsible for activities such as database management, creation of files, bibliographical management and the answering of emergency and routine calls.
A laboratory for veterinary toxicological analysis is linked to the Centre. Its role is to detect, identify and measure the toxic compounds responsible for animal poisonings using biological samples supplied. The relationship between the laboratory and the Centre is very important, as it allows analytical confirmation that a suspected agent was indeed responsible for the poisoning. The laboratory also acts as an official unit for dealing with the poisoning of wild animals; samples from these animals are sent to Lyons from all over France and the epidemiological data obtained is added to existing documentation held at the Centre.

The Centre offers a specialized computerized database that is available to veterinary surgeons via the Minitel telephone information system. More than 500 files or monographs on toxic substances have been established and are available for reference.

The Centre also has an emergency service to deal with serious or significant cases of animal mortality, which only comes into operation when activated by state veterinary departmental services or other official organizations.

Since 1981 all the case data recorded at the Centre and Laboratory have been computerized. Special attention is paid to evaluation of these data. When a call is received, an attempt is made to assess whether the agent mentioned is actually responsible for the poisoning and a 'probability factor' is applied. The bibliography is also computerized and an information specialist is responsible for the acquisition and management of all case data.