- For isolation of CCV or parvovirus in cell culture, fresh faecal material should be placed in virus transport medium and submitted immediately by first class post to a specialist laboratory. Faecal samples must be collected as soon as possible after onset of clinical signs.
- While some viruses grow rapidly from some samples, it may take several weeks before a formal laboratory result is obtained for attempted isolation.
- Remember that CPV can be difficult to detect in faeces once clinical signs have developed, and that CCV is labile and so samples must arrive at the laboratory quickly.
- Feacal samples can also be examined by electron microscopy for virus particles. This technique is especially useful for rotavirus which can be difficult to grow in cell culture. Immunoconcentration may be a useful technique for the detection of parvovirus.
- CPV can also be detected in faeces by haemagglutination or by using commercially available antigen-detection kits. The CPV antigen-capture CITE assay marketed by Iddex Corp. appears to be both sensitive and specific compared with haemagglutination and isolation, and can also detect feline parvovirus in faeces from some cases of feline panleucopenia.
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